{"id":1522,"date":"2025-04-24T06:42:24","date_gmt":"2025-04-24T06:42:24","guid":{"rendered":"https:\/\/medpharma12.com\/?p=1522"},"modified":"2025-05-14T06:40:02","modified_gmt":"2025-05-14T06:40:02","slug":"qualitative-analysis-of-unknown-organic-compound-s-by-lassaign-tests-nitrogen","status":"publish","type":"post","link":"https:\/\/medpharma12.com\/index.php\/2025\/04\/24\/qualitative-analysis-of-unknown-organic-compound-s-by-lassaign-tests-nitrogen\/","title":{"rendered":"Qualitative analysis of unknown organic compound\/s by lassaign tests (Nitrogen)."},"content":{"rendered":"

\u00a0EXPERINMENT 03<\/strong><\/span><\/p>\n

Object<\/strong><\/span><\/p>\n

Qualitative analysis of unknown organic compound\/s by lassaign tests (Nitrogen).<\/span><\/p>\n

Reference<\/strong><\/span><\/p>\n

    \n
  1. Vogel Arthere, Elementary practical organic chemistry, 2nd edition, 1966, published by CBS publisher, page No. 35<\/span><\/li>\n
  2. Agarwal O.P., Practical Organic chemistry, published by Krishna educational publisher, Edition 2014, page no. 24-27.<\/span><\/li>\n<\/ol>\n

    Requirement<\/strong><\/span><\/p>\n

    Ignition tube, capillary tube, pipette, test tube, china dish, filter paper, funnel, unknown organic compound (urea)<\/span><\/p>\n

    Theory<\/strong><\/span><\/p>\n

    The Lassaigne’s test, also known as the sodium fusion test, is a qualitative analysis technique used to detect the presence of nitrogen, sulfur, and halogens in an organic compound.<\/span><\/p>\n

    it utilizes the reactivity of sodium with the elements nitrogen, sulfur, and halogens in organic compounds. When heated with sodium, these elements form water-soluble salts, which are then extracted and tested using specific chemical reactions.<\/span><\/p>\n

    \"\"<\/p>\n

    Procedure<\/strong><\/span><\/p>\n

    Sodium Fusion Test (Lassaigne\u2019s Test)<\/span><\/p>\n

      \n
    1. Take a small piece of dry sodium metal in a fusion tube and heat it gently till the metalmelts or fuses.<\/span><\/li>\n
    2. Add equal quantity of compound to this fused metal [ If he compound is a liquid then add two drops of it with a capillary]<\/span><\/li>\n
    3. Heat it gently then strongly till it becomes red hot.<\/span><\/li>\n
    4. Plung the red hot tube in 10 ml or \u00be of a test tube of distilled water taken in a porcelain dish,covering it immediately with an asbestos sheet crush the fusion tube completely.<\/span><\/li>\n
    5. Carry out more fusion in the similar way. Boil the extract for five minutes reduce the volume to about 5 ml and filter. Perform following test using this filtrate.<\/span><\/li>\n
    6. 1 ml of extract + 2-3 drops of NaOH solution to make it alkaline.<\/span><\/li>\n
    7. Add a few drops of freshly prepared FeSO4solution ,boil for a few minutes ,cool and acidify it with by adding dil. HCl or dil. H2SO4.<\/span><\/li>\n
    8. Blue or green colour solution or Prussian blue coloration.<\/span><\/li>\n<\/ol>\n

      Inference<\/strong><\/span><\/p>\n

      If Prussian blue color is present —–\u00e0 Nitrogen is present<\/span><\/p>\n

      Result:<\/strong><\/span><\/p>\n

      Qualitative analysis of unknown organic compound\/s by lassaign tests (Nitrogen) was done successfully.\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

      \u00a0EXPERINMENT 03 Object Qualitative analysis of unknown organic compound\/s by lassaign tests (Nitrogen). Reference Vogel Arthere, Elementary practical organic chemistry, 2nd edition, 1966, published by CBS publisher, page No. 35 Agarwal O.P., Practical Organic chemistry, published by Krishna educational publisher, Edition 2014, page no. 24-27. Requirement Ignition tube, capillary tube, pipette, test tube, china dish, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":"","_glsr_average":0,"_glsr_ranking":0,"_glsr_reviews":0},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/medpharma12.com\/index.php\/wp-json\/wp\/v2\/posts\/1522"}],"collection":[{"href":"https:\/\/medpharma12.com\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/medpharma12.com\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/medpharma12.com\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/medpharma12.com\/index.php\/wp-json\/wp\/v2\/comments?post=1522"}],"version-history":[{"count":4,"href":"https:\/\/medpharma12.com\/index.php\/wp-json\/wp\/v2\/posts\/1522\/revisions"}],"predecessor-version":[{"id":1571,"href":"https:\/\/medpharma12.com\/index.php\/wp-json\/wp\/v2\/posts\/1522\/revisions\/1571"}],"wp:attachment":[{"href":"https:\/\/medpharma12.com\/index.php\/wp-json\/wp\/v2\/media?parent=1522"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/medpharma12.com\/index.php\/wp-json\/wp\/v2\/categories?post=1522"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/medpharma12.com\/index.php\/wp-json\/wp\/v2\/tags?post=1522"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}